A) How many proteins are being aligned in this analysis? B) What is the name of

Question

A) How many proteins are being aligned in this analysis?

B) What is the name of a bioinformatics tool that is commonly used to produce this alignment output?

C) What is the percentage identity between the rat and lettuce proteins in this aligned region? Show your calculation.

D) Describe ONE thing that a researcher could learn about the gorilla protein from an alignment of this type?

E) Residue 10 shown in each of the sequences is a K or an I. Is K to I a conservative or non-conservative change? Why? Draw structures of the amino acid side chains and explain their properties as part of your answer.

The diagram below shows a multiple protein sequence alignment. Rat ATGGVKHRY RPGTVALREIRRY 50 MARTKOTARKSTGGKAPRKOLATKAARP. Horse ATGGVKHRY RPGTVALREIRRY 50 MARTKOTARKSTGGKAPRKOLATKAARP. Gorilla 1 MARTKOTARKSTGGKAPRKOLATKAARP. RPGTVALREIRRY 50 ATGGVKHRY Worm 1 MARTKOSARISTGGKAPKKOLATKGARPAAGGVKHRYR PGTVALREIRRY 50 Fluke MARTKOSARISTGGKAPRKOLATK MARP. RPGTVALREIRRY 50 ATGAVKHRY Lettuce 1 MARTKOSARISTGGKAPKKOLATKGARPATGAVKHRFR PGTVAL REIRKY 50

Explanation / Answer

A) one protein from six different species aligned.

B) BLAST: Basic Local Alignment Search Tool.(protein). or pBLAST.

C) there are 7 different amino acid between rat and lettuce protein. total 50 amino acid = 100 %. means each amino acid difference = 2% difference.

so 7 different amino acids = 14 % difference.

and similarity = 100 % - 14 % = 86 %.

D) protein from rat, horse and gorilla are 100 % identical.

E) NH2- (CH2)4 - CH (NH2)- COOH = K.(Essential - Proteogenic - Glycogenic and Ketogenic - Basic Side Chain)

CH3- CH2-CH (CH3)- CH (NH2)- COOH = I.(Essential - Proteogenic - Glycogenic and Ketogenic
Non-Polar, Hydrophobic - Aliphatic)

Related Questions

Navigate

• Browse (All)
• Browse A
• Subjects

---

---

Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.