1. Your lab partner wants to complete the lab exercises faster and decides to co

Question

1. Your lab partner wants to complete the lab exercises faster and decides to combine two of the experiments. She decides to test for the optimal pH of the experiment while varying the substrate concentration. She uses the lowest substrate concentration (0.10 mMolar) in the same tube she sets up at pH 3. The next highest substrate concentration (0.50 mMolar) was tested at a pH of 4 and so on. What are the disadvantages to her strategy? What usable information is she likely to obtain from this approach?

Explanation / Answer

The reseacher wants to test the optimal pH of the solution. Optimal pH cannot be checked if she is varying the substarate concentration because only one variable can be checked at a time. Other factors need to be constant while checking the pH, for example, substrate concentration and temperature.

Hence, only either of the two solutions must be taken into consideration (either 0.10 M or 0.50 M). She can also consider any concentration between the two.

Then she should check the optimum pH, by checking the solution gradients of pH. Therefore, she should ensure that only one variable is varying i.e optimal pH. If more than one factors are considered an experimental error would occur.

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