2. In lecture we discussed the intrinsic terminator for the termination step in

Question

2. In lecture we discussed the intrinsic terminator for the termination step in the transcription cycle. However, about half of the termination sites in E. coli lack intrinsic terminators. It was discovered that Rho factors induce the termination of the transcription cycle. What is not a property of Rho factors?

Select one:

a. None of the above

b. Rho requires a recognition sequence on the nascent transcript

c. Rho is a helicase that only unwinds RNA-DNA and RNA-RNA helices

d. Rho is dependent only on ATP hydrolysis

e. Rho travels along a single strand of RNA only in the 5’ to 3’ direction

3. Which is not a likely explanation for abortive initiation?

Select one:

a. The superhelical strain ahead of the RNAP

b. The weak association of the nascent transcript with the template strand

c. The flexible sigma3-sigma4 linker not positioned favorably for transcript elongation

d. The beta-flap binds to the sigma3-sigma4 linker with high affinity

Explanation / Answer

2. The correct answer is A because all other options are true for Rho factors.

3. The correction answer is C because sigma3-sigma4 linker are actually posiitioned for stable transcript elongation

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