please answer with explanation Name(Print):_________TA:__________ Date/Section :
ID: 833413 • Letter: P
Question
please answer with explanation
Name(Print):_________TA:__________ Date/Section :___________ 2 A group of students in your biochemistry jab is interested in amplifying the DNA sequence below. Your job is to come up with a details step-by step PCR procedure to help them. Remember, be specific! Hints: First, you must design both forward and reverse primers. Then, list the materials needed for each step(buffer, dNTPs, DNA, ect?). In addition, draw three major steps of the PCR addition, draw three major steps of the PCR reaction. Again, be specific! 5' AAA CCC GGG TTT AAA CCC GGG TTT AAA CCC GGG TTT AAA CCC GGG 3' 3' TTT GGG CCC AAA TTT GGG CCC AAA TTT GGG CCC AAA TTT GGG CCC 5'Explanation / Answer
.CGCG TTG CAGCTACTGACTC CCC CGCG...
...GCGC AAC GTCGATGACTGAG GGG GCGC...
...CGCG TTG CAGCTACTGACTC CCC CGCG...
AAC
CCC
...GCGC AAC GTCGATGACTGAG GGG GCGC...
...CGCG TTG CAGCTACTGACTC CCC CGCG...
AAC GTCGATGACTGAG GGG GCGC...
...CGCG TTG CAGCTACTGACTC CCC
...GCGC AAC GTCGATGACTGAG GGG GCGC...
...CGCG TTG CAGCTACTGACTC CCC CGCG...
AAC GTCGATGACTGAG GGG GCGC...
CCC
AAC
...CGCG TTG CAGCTACTGACTC CCC
...GCGC AAC GTCGATGACTGAG CGG GCGC...
...CGCG TTG CAGCTACTGACTC CCC CGCG...
AAC GTCGATGACTGAG GGG GCGC...
TTG CAGCTACTGACTC CCC
AAC GTCGATGACTGAG GGG
...CGCG TTG CAGCTACTGACTC CCC
...GCGC AAC GTCGATGACTGAG CGG GCGC...
...CGCG TTG CAGCTACTGACTC CCC CGCG...
AAC GTCGATGACTGAG GGG GCGC...
TTG CAGCTACTGACTC CCC
AAC
CCC
AAC GTCGATGACTGAG GGG
...CGCG TTG CAGCTACTGACTC CCC
...GCGC AAC GTCGATGACTGAG CGG GCGC...
...CGCG TTG CAGCTACTGACTC CCC CGCG...
AAC GTCGATGACTGAG GGG GCGC...
TTG CAGCTACTGACTC CCC
AAC GTCGATGACTGAG GGG
TTG CAGCTACTGACTC CCC
AAC GTCGATGACTGAG GGG
...CGCG TTG CAGCTACTGACTC CCC
...GCGC AAC GTCGATGACTGAG CGG GCGC...
TTG CAGCTACTGACTC CCC
AAC
CCC
AAC GTCGATGACTGAG GGG
TTG CAGCTACTGACTC CCC
AAC
CCC
AAC GTCGATGACTGAG GGG
TTG CAGCTACTGACTC CCC
AAC GTCGATGACTGAG GGG
TTG CAGCTACTGACTC CCC
AAC GTCGATGACTGAG GGG
TTG CAGCTACTGACTC CCC
AAC GTCGATGACTGAG GGG
TTG CAGCTACTGACTC CCC
AAC GTCGATGACTGAG GGG
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