Four pairs of primers were used for PCR amplification of DNA from a patient\'s s

Question

Four pairs of primers were used for PCR amplification of DNA from a patient's somatic cells and from 10 single sperm that he provided for this research. Each of these primer pairs amplifies distinct human SNP loci referred to as A, B, C and D. The four pairs of PCR primers were used on each sample of DNA. Each of the amplified DNAs was divided into ten identical sub-fractions, and these sub-fractions were denatured and spotted onto ten nitrocellulose membrane strips. Each of these strips was then hybridized with two Allele-Specific Oligonucleotide (ASO) specific to each SNP. There are two different ASOs for each of the four SNP loci. Oligonucelotides A1, A2, B1, B2, C1, C1, D1, and D2 reveal single nucleotide differences for each locus. A black circle indicates that the amplified DNA hybridized to the ASO probe Which locus is heterozygous for the alleles tested? A. A B. B C.C D. D E. All are homozygous F. None

Explanation / Answer

Answer is both b and d

An allele specific oligonucleotide or ASO is a short piece of DNA that is complementary to the sequence of a variable target DNA.In the above assay the amplified DNA hybridised to the probe are heterozygous for b as well as d.

Related Questions

Navigate

• Browse (All)
• Browse F
• Subjects

---

---

Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.