You wish to prepare solutions for estimating the Em (molar extinction coefficien

Question

You wish to prepare solutions for estimating the Em (molar extinction coefficient) of an unknown compound.
The compound has a molecular weight of 516 g/mole, is 75.3% pure, and has a molar extinction coefficient of 3000 M-1cm-1.

1. Now you want to make 5.00 mL of a solution that will have an absorbance of 3.00 when measured in a 1 cm pathlength cuvet.
What concentration, in mM, of the compound would you need to make this sample?

2. What volume, in mL, of the stock solution prepared above would be needed to make this sample?

Explanation / Answer

You wish to prepare solutions for estimating the Em (molar extinction coefficien

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