You wish to produce a human enzyme, protein A, by introducing its gene into bact

Question

You wish to produce a human enzyme, protein A, by introducing its gene into bacteria. The genetically engineered bacteria make large amounts of protein A, but it is in the form of an insoluable aggregate with no enzymatic activity. Would the following procedures help you to obtain soluble, enzymatically active protein? Why or Why not?

A. Make the bacteria synthesize protein A in smaller amounts.

B. Dissolve the protein aggregate in urea, the dilute the solution and gradually remove the urea.

C. Treat the insoluble aggregate with protease.

D. Make the bacteria overproduce chaperone proteins in addition to protein

E. Heat the protein aggregate to denature all proteins, then cool the mixture.

Explanation / Answer

The steps are not correct as providing heat will not make the enzyme active.

Also treating the enzyme with protease might break it into fragments.

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