You wish to make 3 reactions (1 ml each) with the specified amounts of protein.

Question

You wish to make 3 reactions (1 ml each) with the specified amounts of protein. The remainder of each reaction consists entirely of buffer. The source of your protein is a stock solution that has a concentration of 0.5 mg/ml. What is the volume of stock solution required for each reaction (column 3). What is the volume of buffer required for each reaction (column 3). What is the colume of buffer required (column 4)? Show your work.
Be sure to pay attention to units.

Reaction Number Amount of protein (ug) Volume of Protein solution (ul) required Volume of buffer required Final Volume (ml) 1 2 1 2 5 1 3 10 1

Explanation / Answer

for reaction 1,

C1V1=C2V2

C1=stock=0.5 mg/ml=500micrograms/ml

V1=?

C2V2=2ug    V1=C2V2/C1=2ug/500ug/ml=0.004ml=4ul

Volume of Protein solution (ul) required=4ul

Volume of buffer required=total volume to be made-0.004ml=1-0.004=0.996ml

for reaction2,

C2V2=5ug

V1=5ug/500ug/ml=0.01ml=10ul

Volume of Protein solution (ul) required=10ul

Volume of buffer required=total volume to be made-0.01ml=1-0.01=0.99ml

for rxn 3

C2V2=10ug

V1=10ug/500ug/ml=0.02 ml=20ul

Volume of Protein solution (ul) required=20ul

Volume of buffer required=total volume to be made-0.02ml=1-0.02=0.98ml

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