Question 4 What is the function of sigma (s) factor? O It binds to the origin of

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Question 4 What is the function of sigma (s) factor? O It binds to the origin of DNA in bacteria, initiating DNA replication. o ltisthe part of the eukaryotic RNA Poymerase that binds to the core promoter resulting in transcription. O lt binds to the bacterial core promoter, activating RNA Polymerase. O lt prevents expression of genes in the lac operon when lactose is not available. Question 5 Which ofthe following is true of RNA transcription but not DNA replication? O Hydrolysis of the monomer triphosphates provides energy. O The polymerase moves along the template from 3 to 5 O The new strand is the reverse complement of the template. O It is fast. O All of the above are true for both replication and transcription. Question 6 Which of the following aspects of transcription is unique to bacteria? Transcription and translation may occur simultaneously. O Only bacteria have mRNAs O RNA splicing. O A 7-methyl guanosine cap at the 5 end of mRNA increases stability. All of the above

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Answer 4: Choice 2 (Sigma factor represents the part of core-protein which helps the RNA polymerase to bind to the promoter by correctly recognizing the promoter binding site. Any impairment in binding of sigma factor to promoter will lead to failure of binding of RNA polymerase to the promoter and thus deterimental for transcription)

Answer 5: Choice 5 (Both transcription and replication are very fast, accurate and high fidelity processes which gain energy from ATP phosphorylation, result in generation of new strand in 3' to 5' direction along with strand complementarity)

Answer 6: Choice 1 (Bacterial transcription might take place simultaneously with DNA replication since bacterial DNA is not packed into histones unlike eukaryotic genome. Thus there is no requirement to open up the chromatin and selectively transcribing it)

Answer 7: Choice 3 (The lactose binds to an active repressor and consequently prevents the masking of the operator in lac operon. This promotes transcription and translation of the operon. Thus, when lactose is present, the active repressor will be present but it will bind to the lactose and undergo inactivation)

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