Question 4 Several things can cause the rate of an enzyme-catalysed reaction to

Question

Question 4 Several things can cause the rate of an enzyme-catalysed reaction to fall away rapidly (i.e. decrease or change), thereby making it difficult to measure an initial rate for purposes of enzyme assay or kinetic substrate assay. Some of these factors include... 1 A back-reaction in which the products are reversibly catalysed back to substrate. 2 Inhibition of the enzyme by one of the products of the reaction. 3 Denaturation of the enzyme at the temperature of the assay. Would the use of a trapping reagent (as frequently used in endpoint assays) be likely to overcome any of the above problems? Include your reasoning. ANSWER (1) YES/NO (2) YES/NO (3) YES/NO

Explanation / Answer

1. Yes: in this case excess substrate is to be used to overcome the problems.

2. No: inhibitor binds the already formed enzyme substrate complex and makes the complex inactive.

3. Yes: Lowering the temperature of the mixture of molecule can make the trapping reagent more effective.

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