The following table shows where different restriction endonucleases (restriction

Question

The following table shows where different restriction endonucleases (restriction enzymes) cleave DNA T A abbreviation R represents the purines (adenine and guanine). The pyrimidines (cytosine, thymine, and abbreviated The abbreviation W represents or thymine. Which restriction endonuclease(s) would cleave a DNA molecule at the following sequences? a) 5'TCAGAATTCGGTGA 3' b) 5'TCCAAGCTTGAATTC 3' EcoRV EcoRI HaeIII HindIII HindIII EcoRI HaeIII EcoRV Plasmid DNA and a gene of interest are cut with the enzyme PpuMI. Write a possible sequence of bases for the sticky end of the gene in the 5' to 3' direction.

Explanation / Answer

a. The answer is
EcoRI - GAATTC - G AATTC

b. The answer is
EcoRI - GAATTC - G AATTC
HindIII - AAGCTT - A AGCTT

The answer for last question is
RG GWCCY

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