DNA methylation is commonly associated with a reduction of transcription. The fo

Question

DNA methylation is commonly associated with a reduction of transcription. The following data come from a study of the impact of the location and extent of DNA methylation on gene activity in human cells. A bacterial gene luciferase. Was cloned next to eukaryotic promoter fragments that were methylated to various degrees, in vitro, The chimeric plasmids were then introduced into tissue culture cells, and the luciferase activity was assayed. These data compare the degree of expression of luciferase with different in the location of DNA methylation (Irvine et al, 2002. Mol. And cell. Boll. 22: 6689-6696). At very high levels of methylation (593 CpGs), which of the following is true? Methylation within the transcription unit has the strongest effect on luciferase expression. Methylation outside the transcription unit has the strongest effect on luciferase expression. Methylation has the same effect regardless of region. None of the above are correct

Explanation / Answer

At very high levels of methylation (593 CpGs), which of the following is true?

c) Methylation has the same effect regardless of region.

As per the data, we observe that at 593 CpGs, the relative luciferase expression is 2x only in both the cases (i.e. methylation at outside or inside transcriptional units). However at lower methylation levels, more inhibition is observed in case of methylation within the transciptional unit.

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