Lysozyme was assayed at bacterial cell wall, [S] = 2.5x10-8 g. The Km for the su

Question

Lysozyme was assayed at bacterial cell wall, [S] = 2.5x10-8 g. The Km for the substrate = 6x10-3g. At the end

of one minute 2.5% of the cell wall hydrolysed. Answer the following questions using this information:

a) What percent of bacetrial cell wall will be hydrolyzed at the end of 3 minutes assuming product formation

is in 1st order of reaction?

b) What will be a concentration of hydrolyzed product at the end of 4 minutes?

c) Determine maximal velocity (Vmax) with the enzyme concentration used.

Explanation / Answer

a)7.5%as first order kinetics is followed 2)2.5 * 10^-9,as 10% of the product is hydrolysed 3)3.4 * 10^13

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