absorbance of a vitamin is measured at 264 nm, it follows Beer\'s law over a wid

Question

absorbance of a vitamin is measured at 264 nm, it follows Beer's law over a wide range of concentrations. e= 16200 M-1 cm-1, cell length = 1cm a) if the absorption has to be between .25 as the low range and .85 as the optimum range, what is the valid range of concentration for this assay? [did this part - just want to check answer] b) You measured an absorbace at 264 nm of 1.2 for your unknown vitamin sample. Can you use this reading to determine the initial concentration of your vitamin? If so, show how you would do this. If not, explain why and propose how you would treat the unknown sample (eg. 1 ml sample + ____ ml diluent)

Explanation / Answer

a) A= ecl 0.25 = 16200 x 1 xC , C = 1.54 x10^ -5 M 0.85 = 16200 xC , C = 5.2 x10^ -5 M so range of conc = (1.54 x10^ -5 M --5.2 x10^ -5M) b) A = 1.2= 16200 xC C = 7.4 x10^ -5 M initial conc of ample can be known only when we know abdorbance at initial

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