able 5.1 PURIFICATION OF A TARGET PROTEIN USING A COMBINATION OF BIOCHEMICAL MET

Question

able 5.1 PURIFICATION OF A TARGET PROTEIN USING A COMBINATION OF BIOCHEMICAL METHODS Total units of activity MutifoldTotal activity increase in yield (%r Specific Totalmultfold Total purification, Protein purification method Preparation of a crude cell extract Subcellular fractionation using (mg) (units/mg). purity. : : 200020,000 18,000 10 100 1.0 500 36 differential centrifugation Ammoniun sulfate precipitation Gel-filtration chromatography lon exchange chromatography Affinity chromatography 3.6 2.5 3.2 2.7 90 3.6 180 45 15 0.4 16,000 13,000 11,500 89 289 767 8.9 28.9 76.7 492.475.0 80 65 57 . 9.90024.750 32.3 Thought Question 15: What was the best method for this purification? a. Subcellular fractionation. b. Ammonium sulfate precipitation. c. Gel filtration chromatography. d. lon exchange chromatography. e. Affinity chromatography

Explanation / Answer

5.1 affinity chromatography.as here the total multifold purification is maximum

17. e. N terminal pKa

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