3. Protein sequencing II. Determine the sequence of a peptide consisting of 14 a

Question

3. Protein sequencing II. Determine the sequence of a peptide consisting of 14 amino acids on the basis of the following data.
o Amino acid composition: (4S,2L,F,G,I,M,T,W,Y, K)
o N-terminal analysis: S
o Carboxypeptidase digestion: L (cuts from the C-terminus, tells you the last amino acid)
o Trypsin digestion: (3S,2L,F,I,M,T,W) (G,K,S,Y)
o Chymotrypsin digestion: (F,I,S) (G,K,L) (L,S) (M,T) (S,W) (S,Y)
o N-terminal analysis of (F,I,S) peptide: S
o Cyanogen bromide treatment: (2S,F,G,I,K,L,M*,T,Y) (2S,L,W)

Explanation / Answer

SYGKLSIFTMSWSL

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