Suppose a scientist identified two yeast mutants, called huh1 and huh2. The muta

Question

Suppose a scientist identified two yeast mutants, called huh1 and huh2. The mutants have constitutive expression of the nop operon, which includes the genes nop1 and nop2. One of the mutants is a point mutation in a DNA-binding site, and the other is a deletion of the gene which encodes the DNA-binding protein.
Which term describes the regulatory function of the DNA-binding protein?
The scientist then performs a experiment mating the two haploid yeast together to determine which mutation is in the DNA-binding protein and which is in the dna-binding site. The experimental setup is listed below, wherein a minus indicates a lose-of-function mutation, and a plus indicates the wild type allele.
Suppose a scientist identified two yeast mutants, called huh1 and huh2. The mutants have constitutive expression of the nop operon, which includes the genes nop1 and nop2. One of the mutants is a point mutation in a DNA-binding site, and the other mutant is a deletion of the gene which encodes the DNA- binding protein. Which term describes the regulatory function of the DNA-binding protein? O negative regulator O not a regulator O positive regulator The scientist then performs an experiment mating the two haploid yeast together to determine which mutation is in the DNA-binding protein and which is in the DNA-binding site. The experimental setup is listed below, wherein a minus indicates a loss-of-function mutation, and a plus indicates the wild-type allele. Experiment Haploid grotype #1 | Haploid genotype #2 huh 1-huh2. nop1" nop2. ) huh! . huh2. nop1" nop2- huht huh2 nop1 nop huh1' huh2 nop1 nop2 2 Continued below... How will nop1 and nop2 be expressed if huh1 encodes the DNA-binding protein and huh2 is a mutation in the binding site? How will nop1 and nop2 be expressed if huh2 encodes the DNA-binding protein and huh1 is a mutantion in the binding site? Experiment1 Experiment 2 Experiment 1 Experiment 2 O Previous Gfvet Up & Niew Sciulion 9 Check Answer O Next Hiot

Explanation / Answer

DNA binding protein is a regulator protein that binds to the regulator sequence in the gene and do not allow RNA polymerase to proceed for transcription so that there is no expression of gene observed. So we can call it as a negative regulator. In operon this is a normal phenomenon and expression dependents on the need of cell for particular component.

In the present experiment there are two genes nop1 and nop2.

The nop operon will have following sequential structure--

gene for DNA binding protein-------site for DNA binding protein---------promoter------nop1-----nop2

+ indicated the wild type allele , hence considering that wild type alleles are dominant following observations can be the outcome. I have summarized it in the table.

huh1 encodes for DNA binding protein

huh 2 is mutation in DNA binding site

1. If huh1 encodes for Dna binding protein and huh2 is mutaion in binding site:

Reason

Experiment 1

Huh1- huh2+

Mutation in binding site hence DNA binding protein cannot bind to the site and there is production of both nop1 and nop2

Huh 1+ huh2+

Both gene and site are mutated henec no regulation of expression . nop1 and nop2 are produced

Experiment 2

Huh1+ huh2-

DNA binding protein is produed but could not bind to binding site and there is production of both nop1 and nop2

Huh1+ huh2+

Both gene and site are mutated henec no regulation of expression . nop1 and nop2 are produced

If huh1 is mutaion in binding site and huh2 encodes for Dna binding protein

Reason

Experiment 1

Huh1- huh2+

Mutation in DNA binding protein hence it cannot bind to the site and there is production of both nop1 and nop2

Huh 1+ huh2+

Both gene and site are mutated hence no regulation of expression . nop1 and nop2 are produced

Experiment 2

Huh1+ huh2-

DNA binding protein is produed but could not bind to binding site and there is production of both nop1 and nop2

Huh1+ huh2+

Both gene and site are mutated henec no regulation of expression . nop1 and nop2 are produced

Reason

Experiment 1

Huh1- huh2+

Mutation in binding site hence DNA binding protein cannot bind to the site and there is production of both nop1 and nop2

Huh 1+ huh2+

Both gene and site are mutated henec no regulation of expression . nop1 and nop2 are produced

Experiment 2

Huh1+ huh2-

DNA binding protein is produed but could not bind to binding site and there is production of both nop1 and nop2

Huh1+ huh2+

Both gene and site are mutated henec no regulation of expression . nop1 and nop2 are produced

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