Suppose a researcher uses a single SELEX experiment to select for an RNA molecul

Question

Suppose a researcher uses a single SELEX experiment to select for an RNA molecule that catalyzes the hydrolysis of a particular ester. Select statements that describe good design parameters for her SELEX experiment.

-A 30 nucleotide RNA is beyond the practical size limit of her SELEX experiment.

-A 10 nucleotide RNA molecule is beyond the practical size limit of her SELEX experiment.

-RNAs with potential hydrolysis activity will be captured by size-exclusion chromatography.

-The chromatographic resin must be bound to an analog of the reactants of the ester hydrolysis reaction.

-A transition-state analog of the ester hydrolysis reaction will be tethered to a chromatographic resin.

Explanation / Answer

SELEX studies can be generally divided into two groups. In the first group, nucleic acid library is predetermined by the task of the study such as for the in vitro selection of ribozymes, riboswitches, DNAzymes or genomic SELEX studies

The second group includes SELEX studies on aptamers that will be further employed for research, therapeutic or bioanalytical purposes

good design parameters for her SELEX experiment ARE:

1)RNAs with potential hydrolysis activity will be captured by size-exclusion chromatography.

2)The chromatographic resin must be bound to an analog of the reactants of the ester hydrolysis reaction.

3)-A transition-state analog of the ester hydrolysis reaction will be tethered to a chromatographic resin.

Related Questions

Navigate

• Browse (All)
• Browse S
• Subjects

Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.