Suppose a scientist identifies a novel human protein. In order to determine the

Question

Suppose a scientist identifies a novel human protein. In order to determine the cell types in which the protein is expressed, the scientist decides to perform a Western blot. A Western blot is an experiment that identifies the presence of specific proteins using antibodies. When the protein is present, an antibody will bind to it and the presence of the antibody can be visualized using chemical reactions. The ladder contains proteins of known sizes in kiloDaltons. Several tissue samples were collected from the skin (Sample A), the livetr (Sample B), the lungs (Sample C), the brain (Sample D), and the smal intestine (Sample E). Which tissues produce this protein? Ladder A B C D E lungs 100 brain liver 75 small intestine skin 50

Explanation / Answer

Since the antibody for the novel human protein was bound to only the samples A and D, the protein must have only been present in these two tissue types.

Hence, the protein is produced by Skin (Sample A) and Brain (Sample D) only.

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