1.0 6. What is the Extinction Coefficient for tryptophan, based on your linear r
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Question
1.0 6. What is the Extinction Coefficient for tryptophan, based on your linear regression analysis? 7. Using the linear regression equation in question 4, find the concentration of the unknown i Tryptopharn 8. What is the published value for tryptophan, in the same units as for the previous question? 1.0 9. Give a short explanation of the Extinction Coefficient and its utilization in Biochemistry. 10. Bibliography (cite at least one reference, it should be a journal paper or a reference book, use USING BRITISH STANDARD HARVARD) 1.0]Explanation / Answer
(6.) The slope of absorbance vs. concentration gives molar absorptivity coefficient, ? x l. Here ? is the molar extinction coefficient and l is path length.
Provided the path leangth l, extinction coefficient = slope / path length
Here slope = 0.5926 and length = 1 cm (assume)
extinction coefficient = 0.5926 mM/cm.
(7.) Question 4 is not given, But the method is as follow: For the given valus of absorbance (y), the concentration (x) can be calculated as:
x (in mM) = (y - 0.0233)/0.5926
(8.) Published paper data are not given.
(9.) Extinction coefficient: It is a proportinality coefficient in expression for Beer Lambert's law for determining the concentration of a substance using colorimeter (spectrophotometers). Beer’s Law states that absorbance of a sample (Abs) depends on the molar concentration (c), light path length in centimeters (l), and molar extinction coefficient (?) for the dissolved substance at the specified wavelength (?). For a measured absorbance value from colorimeter methids, the concentration of chemical or biological molecule in a solution can be obtained.
This method of protein amino acid analysis is a quantitative non-destructive method used in biochemistry.
(10.) Edelhoch, H., "Spectroscopic determination of Tryptophan and Tyrosine in Protein" Biochemistry, 1967, 6 (7), pp 1948–1954.
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