C Spire LTE 10:59 PM Back Final Exam 2016.pdf A constant concentration of substr

Question

C Spire LTE 10:59 PM Back Final Exam 2016.pdf A constant concentration of substrate was incubated with a constant concentration of enzyme for various time intervals. Determine the initial reaction rate for a enzyme catalyzed reaction using the data bekow Note: The absorbance readings over the time peried of O min to 10 min in the absence of enryme produced absorbance readings of zcro for all time periods 10 5.0 10.0 15.0 17.5 20.5 02 810 12 Time, min (a) 2.1 nmol/min (b) 2.5 nmol min c)1.0 nmol min (d) 5.0 nmol/ min Questions 9-10 Use the data below for ans enzyme catalyzed reaction. The total enzyme concentration fer the reaction is S.0 nM Calendar To Do Notifications Inbox

Explanation / Answer

8. For the given reaction data

initial reaction rate will be calculated between 0 to 4 min

so,

between 2 and 4 min

with [A] being amount of substrate remaining

[A] at t = 2 min = 100 - 5 = 95 mmol

[A] at t = 4 min = 100 - 10 = 90 mmol

we get,

initial reaction rate = -change in concentration/time

                              = -(90 - 95)/(4 - 2)

                              = 2.5 mmol/min

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