You are interested to prepare a 50 ml agarose gel that has a final concentration

ID: 67885 • Letter: Y

Question

You are interested to prepare a 50 ml agarose gel that has a final concentration of 2% (w/v) agarose and 1X SYBR Safe Stain. How much agarose (in grams) and 10,000X SYBR Safe Stain (in microliters) will you use to prepare this gel?

You are interested to prepare a 50 ml agarose gel that has a final concentration of 2% (w/v) agarose and 1X SYBR Safe Stain. How much agarose (in grams) and 10,000X SYBR Safe Stain (in microliters) will you use to prepare this gel?

You are interested to prepare a 50 ml agarose gel that has a final concentration of 2% (w/v) agarose and 1X SYBR Safe Stain. How much agarose (in grams) and 10,000X SYBR Safe Stain (in microliters) will you use to prepare this gel?

Explanation / Answer

1) 2% means 2g in 100ml

We need to prepare 50 ml of agarose gel solution,

So, we need= 2g/2=1g.

So 1 g of agarose is needed to prepare 2% of 50ml agarose gel

2) Preparation of 1X SYBR Safe Stain for 50 ml of agarose gel solution

Dilute 5 µL of 10,000X SYBR Safe Stain concentrate with 50 ml buffer and you will get 1X SYBR safe stain for 50ml agarose gel.

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You are interested to prepare a 50 ml agarose gel that has a final concentration

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