You are interested in studying eukaryotic transcription factors and have develop

Question

You are interested in studying eukaryotic transcription factors and have developed an in vitro transcription system using a segment of DNA that includes a transcribed gene and its predicted upstream regulatory region (see figure below). The transcription of this DNA occurs in a defined system in which you add purified RNA polymerase II and basal factors You perform a series of experiments that compare the efficiency of transcription in your defined system with the efficiency of transcription in a crude nuclear extract. You test the two systems with your WT DNA and various deletion mutants that you have generated (see figure below). The results of your study are shown in the table:

Explanation / Answer

The defined system might be lacking in coactivators, chromatin remodelers, histone acetylases, deacetylases, kinases, and methylases. Basal transcription factors alone cannot ensure the efficiency of transcription a large number of transcription factors apart from the basal factors are necessary for efficient transcription.

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