Shown below is a cDNA fragment containing the coding sequence (the start and sto

Question

Shown below is a cDNA fragment containing the coding sequence (the start and stop codons are underlined) for the Aspergillus fumigatus ?-amylase. Use this sequence to answer the following questions (letter your responses appropriately).
(A) The restriction endonuclease MspI recognizes the sequence C ? CG ?G, and cleaves the DNA as shown. How many times will MspI cut the given sequence? [1 pt]
(B) What are the nucleotide positions of the cleavage events? [1 pt]
(C) How many DNA fragments will be produced by MspI cleavage? [1 pt]
(D) What is the size of each DNA fragment produced? [1 pt]

Explanation / Answer

(A) The restriction endonuclease MspI recognizes the sequence C CG G, and cleaves the DNA as shown. How many times will MspI cut the given sequence?

At 3 places, the MspI will cleave.

(B) What are the nucleotide positions of the cleavage events?

First cleavage 186-189.

Second cleavage 244-247 (247-244).

Third cleavage 537-540.

(C) How many DNA fragments will be produced by MspI cleavage?

4 DNA fragments

(D) What is the size of each DNA fragment produced?

185, 57, 234 and 664.

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