Shown below are the absorption and fluorescence spectra for an antibody in pH 7.

Question

Shown below are the absorption and fluorescence spectra for an antibody in pH 7.2 buffer: Which is the fluorescence spectrum, the red one or the blue one? Explain. What is the best wavelength at which to excite this sample in order to study its fluorescence? Explain. What is the best wavelength at which to monitor the fluorescence of this sample for quantitative purposes? Explain. What is the longest wavelength at which the same could be excited and still produce its entire fluorescence spectrum? Explain.

Explanation / Answer

(a) Red spectrum is fluorescence spectrum since fluorensce alway come more than absorption.

(b) 550 nm is the best wavelength to study the experiment

(c) 580 nm is the best wavelength for quantitative purpose

(d) 580 nm is the longest wavelength coul be excited and still produce its entire fluorescence spectrum.

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