3. The sequence of nucleotides in an mRNA is: 5’-CCAUGACCCAUUGGUCUCGUUAGGA-3’ A)

Question

3. The sequence of nucleotides in an mRNA is: 5’-CCAUGACCCAUUGGUCUCGUUAGGA-3’

A) Assuming that ribosomes could translate this mRNA, how many amino acids long would you expect the resulting polypeptide chain to be?

B) Hydroxylamine, which causes C/G!T/A transitions, is applied to cells that make this mRNA molecule. A new cell line is isolated in which a mutation occurred at the 13th position of the DNA that coded for the mRNA. Draw a diagram that clearly illustrates the idea that 2 rounds of DNA replication must occur in order to permanently introduce this mutation into the genome.

C) How many amino acids long would you expect the polypeptide made by this mutant cell line to be?

Explanation / Answer

5’-CCA UGA CCC AUU GGU CUC GUU AGG A-3’

A) If this mRNA could translate then it will produce 6 amino acids because there are 8 triplet codons present in this mRNA, but the second one is the stop codon. So, only the polypeptide could be 6 amino acids long.

B) 5’-CCAUGACCCAUU- 3’

     3’ GGUCUCGUUAGGA-5’

C) 1 amino acids long protein can be produced.

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