Your friend is studying a protein of 343 amino acids localized to secretory vesi

Question

Your friend is studying a protein of 343 amino acids localized to secretory vesicles. She wants to define the regions of the protein responsible for its localization to this particular class of vesicles. She creates a series of deletion constructs in which she deletes different amino acid segments from the protein. She expresses each of her proteins in cells and monitors the proteins’ localizations within the cell, obtaining the data depicted in Figure 1.

Figure 1

A. From these data, where do you predict the localization sequence is for this protein?

B. If your friend made a protein that lacked only those sequences you defined in part A and if she expressed this protein in cells, where would you predict that it would end up? Explain.

Explanation / Answer

A. If the segment between 149-268 is absent then protein translocation is not taking place. So, 149-268 is the localization sequence in the protein.

B. since the protein has no localization sequence, then the protein would be synthesized and end up in the cytoplasm.

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