Your friend is studying a protein of 343 amino acids localized to secretory vesi
ID: 62930 • Letter: Y
Question
Your friend is studying a protein of 343 amino acids localized to secretory vesicles. She wants to define the regions of the protein responsible for its localization to this particular class of vesicles. She creates a series of deletion constructs in which she deletes different amino acid segments from the protein. She expresses each of her proteins in cells and monitors the proteins' localizations within the cell, obtaining the data depicted in Figure 1. A. From these data, where do you predict the localization sequence is for this protein? B. If your friend made a protein that lacked only those sequences you defined in part A and if she expressed this protein in cells, where would you predict that it would end up? Explain.Explanation / Answer
A. The localization sequence lies between 149 - 268 as deletion of this region abrogates the protein's localization to the secretory vesicles.
B. The protein would end up in the Golgi. The secretory pathway involved in protein trafficking involves ER-Golgi. The proteins first enters ER followed by Golgi and are sorted to different destinations from Golgi. Since deletion of this region also abrogates transit through Golgi the protein would most likely end up in Golgi when it lacks the proper localization sequences.
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