1.) oxidative phosphorylation is the process in mitochondria by which: A.Electro
ID: 63183 • Letter: 1
Question
1.) oxidative phosphorylation is the process in mitochondria by which:
A.Electrons reduce O2 to H2O and this causes ATP to be made.
B.Glycolysis produces more ATP than is needed to activate its pathway.
C.Oxygen is used to cause phosphorylation of biological molecules.
D.Synthesis of ATP is dependent on the passage of electrons through the electron transport chain.
2.) You incubate isolated intact mitochondria in a buffered solution containing succinate (an oxidizable substrate) and ADP plus Pi. Upon adding cyanide (an inhibitor of Complex IV, cyto-chrome oxidase), you examine the effect on oxygen consumption and the production of ATP. What do you predict?
A.Oxygen will not be consumed, and no ATP will be produced.
B.Oxygen will be consumed, but no ATP will be produced.
C.Oxygen will not be consumed, but ATP will be produced.
D.Oxygen will be consumed, and ATP will be produced.
3.) You incubate isolated intact mitochondria in a buffered solution with succinate (an oxidizable substrate) and ADP plus Pi. Upon adding oligomycin, an antibiotic inhibitor of ATP synthase, you examine the effect on oxygen consumption and the production of ATP. What do you predict?
A.Oxygen will not be consumed, and no ATP will be produced.
B.Oxygen will be consumed, but no ATP will be produced.
C.Oxygen will not be consumed, but ATP will be produced.
Oxygen will be consumed, and ATP will be produced
4.) You incubate isolated intact mitochondria in a buffered solution with succinate (an oxidizable substrate) and ADP plus Pi. You add oligomycin (the ATP synthase inhibitor), then the compound DNP (2,4-dinitrophenol), and examine the effect on oxygen consumption and the production of ATP. What do you predict? Hint: What happened when people took this as a weight-loss drug?
A.Oxygen will not be consumed, and no ATP will be produced.
B.Oxygen will be consumed, but no ATP will be produced.
C.Oxygen will not be consumed, but ATP will be produced.
D.Oxygen will be consumed, and ATP will be produced.
5.) You have created some artificial membrane vesicles (spherical lipid-bilayer enclosed droplets) that have a higher pH inside than the aqueous solution outside. To sample 1 of these vesicles you add a little HCl, and to the other (sample 2) you add some DNP (with equivalent acidity) and then measure the internal pH of the vesicles. What do you predict?
A. Internal pH will be lowered for both 1 and 2.
B. Internal pH will be unchanged for both 1 and 2.
C. Internal pH will be unchanged for 1 but lowered for 2.
D. Internal pH will be lowered for 1 but unchanged for 2
6.) You isolate intact mitochondria and equilibrate them in a buffered solution at pH 9, containing 0.1 M KCl and ADP plus Pi, but without succinate. You collect them by centrifugation, and quickly resuspend them in a new buffer at pH 7, without KCl , but with valinomycin (a K+ ionophore). Note: the K+ rushing out will create a huge positive charge differential. What do you predict will be the result on oxygen consumption and the production of ATP?
A. Oxygen will not be consumed, and no ATP will be produced.
B. Oxygen will be consumed, but no ATP will be produced.
C. Oxygen will not be consumed, but ATP will be produced.
D. Oxygen will be consumed, and ATP will be produced.
Explanation / Answer
1. D.Synthesis of ATP is dependent on the passage of electrons through the electron transport chain.
“Oxidative phosphorylation” is an aerobic process that involves a series of reactions, which include the transfer of electrons from the NADH or FADH2to oxygen. This process involves the transfer of electrons (oxidation) by a series of electron carriers (electron transport system (ETS)) and attachment of phosphate group (phosphorylation).
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