A growth factor for cells was identified from extracts of rat brain. Preliminary

Question

A growth factor for cells was identified from extracts of rat brain. Preliminary characterization was done on this activity using several methods. Less than 5% of the activity could be precipitated by 80 % ammonium sulfate @ pH 7.5. Heat treatment ( 100 C for 10 minutes) had no effect on biological activity. On isoelectric focusing, the apparent isoelectric point of the active material was 4.8. By electrophoresis the growth factor was found to have a MW= 21,000. A. Based on the information given above, is this growth factor a protein? Explain. B. What other test could you do to confirm your answer in part a?

Explanation / Answer

we can do other test like SPECTROSCOPY which will give a particular peak and crytolography or chromasin binding blue to identify its a protein or not....as the isoelectic point is 4.8 and by electrophorosis I.E its a charged molecule ..and about the heat tratment there are some proteins which can withstand high tem ..hence it could be a protein

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