DNA METHODS & POPULATION GENETICS QUESTION Please reference all information if t
ID: 63023 • Letter: D
Question
DNA METHODS & POPULATION GENETICS QUESTION
Please reference all information if taken from the Internet. Paraphrase and send me the link as well. Thanks!
1. Indels, which are polymorphic insertions and deletions in the genome, are the latest marker system being used by the forensic community. Recently an Indel commercial kit, known as DIPplex, has been released. Please fully describe this marker system, both in and outside the context of the commercial kit- how does it work? Do these differ from ALUs- why or why not? Finally, what is your opinion their future in forensics. (10 pts)Explanation / Answer
The Investigator DIPplex Kit enables the amplification of 30 deletion/insertion polymorphisms (also known as INDELs) plus Amelogenin. This provides an equally sensitive alternative to STR- and SNP-based human identification. The kit is designed to analyze highly degraded DNA, thanks to the maximum amplicon size of just ~160 bp. The genetic analysis is calibrated with BT5 Matrix, an optimized dye technology.
Marker sets that are based on small insertion/deletion (INDEL) alleles can serve as useful supplementary or stand-alone assays for human identification. A validation study has been performed on a human identification assay based on a panel of 30 INDELs and amelogenin using the Investigator DIPplex® kit (Qiagen). The assay was able to type DNA from a number of forensically relevant sample types and obtain full profiles with 62 pg of template DNA and partial profiles with as little as 16 pg of template DNA. The assay is reproducible, precise, and non-overlapping alleles from minor contributors were detectable in mixture analysis ranging from 6:1 to 19:1 mixtures. Population studies were performed on the 30 indels, and there were no significant departures from Hardy-Weinberg equilibrium or significant linkage disequilibrium between the markers (after correction for sampling). In all populations, the random match probability was 1.43×10(-11) or less, and the power of exclusion was greater than .999999999. We also discovered several microvariant alleles in our population samples. The data support that the Investigator DIPplex® kit provides a powerful supplement or stand-alone capability for human identity testing.
http://www.cstl.nist.gov/strbase/pub_pres/ISHI2011-InDel.pdf (THIS PDF MAY HELP YOU IN UNDERSTANDING)
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