DNA METHODS & POPULATION GENETICS QUESTION Please reference all information if t
ID: 63021 • Letter: D
Question
DNA METHODS & POPULATION GENETICS QUESTION
Please reference all information if taken from the Internet. Paraphrase and send me the link as well. Thanks!
As Lucinda Luciano pondered the age old question, was it better to be good, or lucky, she tried to stop the bleeding with the only thing she could find nearby ?a few crumbled up Kleenexes from her coat pocket. Days later, Detective Ridgeway, the lead investigator from the missing person?s squad, recovered the blood-stained discarded tissues in a garage stairwell. What could these possibly disclose? He was sure a genetic profile could be created, but a profile of whom: the assailant, the victim, or from an event unrelated to the crime? You and your partner have been sent the spent Kleenexes. Can you establish a date for when the Kleenexes where discarded? What would this reveal? In order to create a genetic profile for the Local, State and Federal agencies, what marker system must you use? What extraction protocol, do you feel most competent with and why? What problems might you encounter using a blooded tissue, rather than a blood sample on a FTA card? Design a research plan, complete with hypotheses that can be answered. (15 pts)Explanation / Answer
If that is the case of investigation, the sample to be collected from the kleenexes to recover the DNA. In case of criminal investigation to know whom the blood stain is, DNA profiling should be done. During DNA profiling, it might be possible to get contamination from the discarded kleenexes while taking sample. In case of forensic investigation DNA finger printing is the best method to prove the person accused is a victim. For that the marker system to be used is Restriction fragment length polymorphism or RFLP. Unrelated people have different short tandem repeats (STR), so PCR connected STR analysis also discriminate the DNA between two unrelated individuals.
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