Transcription and Translation Worksheet 1. The DNA sequence 5’-T T A A C G G C T
ID: 60994 • Letter: T
Question
Transcription and Translation Worksheet 1. The DNA sequence 5’-T T A A C G G C T T T T T T C G T A C A T-3’ was used as a template to synthesize a molecule of mRNA that was then translated into protein.
A. Give the sequence of each of the following AND indicate the 5’ and 3’ ends of each.
DNA coding strand –
DNA template strand –
mRNA sequence –
B. Translate your mRNA molecule into a polypeptide chain using the genetic code.
C. Indicate the C-terminal amino acid and the N-terminal amino acid of the resulting polypeptide.
2. Draw a simple diagram showing where transcription, RNA modification/splicing, and translation occur in the cell. 3. Given the DNA sequences below of coding strands, identify and underline the mutation in each. What effect did each DNA mutation have on the final protein products. What type of mutation occurred (synonymous, nonsynonymous, nonsense, nonstop)? Explain. Original DNA Sequence
5’ – A T G T T T C T G G T C A A T T G C G C A G G T C C A A C C T A G – 3’
Mutated Sequences A. 5’ – A T G T T T T T G G T C A A T T G C G C A G G T C C A A C C T A G – 3’
B. 5’ – A T G T T T G T G G T C A A T T G C G C A G G T C C A A C C T A G – 3’
C. 5’ – A T G T T T C T G G T C A A T T G C G C A G G T C C A A C C T A A – 3’
D. 5’ – A T G C T T T C T G G T C A A T T G C G C A G G T C C A A C C T A G – 3’
E. 5’ – A T G T T T C T G G T C A A T T A G C G C A G G T C C A A C C T A G – 3’
F. Which of the above mutations do you think would be more deleterious to the cell/organism? Explain your reasoning. 4. Would a random mutation in a coding region of the genome or a random mutation that occurs during transcription/mRNA processing be worse for the cell/organism overall? Explain.
Explanation / Answer
1.
B. Polypeptide chain – LTAFFVH
C. N’- LTAFFVH-C’
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