Transcription and Translation Questions 6. The template DNA sequence is 5\' ACCT

Question

Transcription and Translation Questions 6. The template DNA sequence is 5' ACCTGAGTC 3', what is the transcribed mRNA sequence from 5' to 3'? Second base Pheny F UCC -Serine UAA UUC alanine UUA UUG Leucine L CUU UCA SUAA Stop codon UGA Stop codon UCG CCU CUA Leucine L CCc UAG Stop codon UGG cCC -ProlneCAA-Glutam CAC CAG AAU Asparagine AGC CAU-Histidine H cGc CGU CGA Arginine CCG AUU AUC Isoleucine AUA AUG IACUu NAGCSerine S K AGGArginine GGC -Glycine ACC-Threonine AAA-Lysine K AGA ethionine ACG AAG GUU GUC GUA Valine V GCU GAU1 Aspartic GGU acid D GAA 1 Glutamic GGA GAC GCA GCG GUG acid EGGG O2011-2016 Birla Institute of Scientific Research 7. Refer to the table above, what are the amino acids that attach to the mRNA in Q6 from N-terminus to C-terminus? 8. When the second C in the template DNA sequence in Q6 mutates to T, mutated DNA is 5' ACTTGAGTC 3', describe the changes in the amino acids synthesis.

Explanation / Answer

Answer:

6). Template DNA can be read from 3’-5’, so we need to rewrite the sequence as follows.

Template DNA---3’ CTGAGTCCA 5’

mRNA-------------5’ GACUCAGGU 3’

7).

mRNA-------------5’ GACUCAGGU 3’

Amino acids----- N’- Asp -Ser –Gly-C’

8).

Template DNA---3’ CTGAGTCTA 5’

mRNA-------------5’ GACUCAGAU 3’

Amino acids----- N’-Asp -Ser –Asp-C’

Amino acid is changed from glycine to aspartic acid. This mutation is called missense mutation.

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