1. A micrococcal nuclease digestion experiment was performed on chromatin from t
ID: 60863 • Letter: 1
Question
1. A micrococcal nuclease digestion experiment was performed on chromatin from two different lines of Drosophilia. Both fly lines contain the same transgene (a piece of DNA that has been experimentally added to the genome), but the chromosomal location of the transgene in each line is different. In the 39C-X line, the micrococcal digestion depicts the transgene with nucleosome arrays of up to 5 nulceosomes . In the HS-2 line, one can clearly see arrays with up to 9 nucleosomes present on the transgene. Given these results, which gene is more likely to be located in euchromatin (“open chromatin”)?
More information is needed.
Neither 39C-X or HS-2 is likely to be located in open chromatin.
39C-X
Both 39C-X and HS-2 are likely located in open chromatin.
HS-2
2. For the partial mRNA sequence shown, what is the corresponding sequence of the non-template strand of the gene from which the mRNA was transcribed?
5’- CCGACUACGGCUACG -3’
5’-CGUAGCCGUAGUCGG-3’
3’-CGTAGCCGTAGTCGG-5’
5’-CCGACTACGGCTACG-3’
5’-CGTAGCCGTAGTCGG-3’
3’-CCGACTACGGCTACG-5’
a.More information is needed.
b.Neither 39C-X or HS-2 is likely to be located in open chromatin.
c.39C-X
d.Both 39C-X and HS-2 are likely located in open chromatin.
e.HS-2
Explanation / Answer
Ans-2 (d)
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