1. A micrococcal nuclease digestion experiment was performed on chromatin from t
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Question
1. A micrococcal nuclease digestion experiment was performed on chromatin from two different lines of Drosophilia. Both fly lines contain the same transgene (a piece of DNA that has been experimentally added to the genome), but the chromosomal location of the transgene in each line is different. In the 39C-X line, the micrococcal digestion depicts the transgene with nucleosome arrays of up to 5 nulceosomes . In the HS-2 line, one can clearly see arrays with up to 9 nucleosomes present on the transgene. Given these results, which gene is more likely to be located in euchromatin (“open chromatin”)? a. More information is needed. b. Neither 39C-X or HS-2 is likely to be located in open chromatin. c. 39C-X d. Both 39C-X and HS-2 are likely located in open chromatin. e. HS-2
Explanation / Answer
In the 39C-X line, the micrococcal digestion depicts the transgene with nucleosome arrays of up to 5 nulceosomes . In the HS-2 line, one can clearly see arrays with up to 9 nucleosomes present on the transgene. Given these results, both 39C-X and HS-2 are more likely to be located in euchromatin (“open chromatin”). Option d is correct. This is because D. melanogaster chromatin generally shows an array of five to seven nucleosomes, this clearly indicates that the pattern observed for the euchromatic transgene holds for the major portion of the genome.
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