Your favorite patient has just had surgery. After surgery, Bactroban was applied

Question

Your favorite patient has just had surgery. After surgery, Bactroban was applied. After a few days, you notice an infection around the incision. Even though more Bactroban is applied, the infection persists. You want to identify the bacteria causing the infection to determine what treatment is best. First, you need to collect a sample.

1. What kind of medium do you place your swab into to grow your bacteria?

2. After incubating for 24 hours, will you have a pure culture? Give an explanation for your answer!

3. What is the next step and what is the purpose of it?

Explanation / Answer

1. MacConkey agar medium

2. No.Pure culture is not seen. Because, it is infected by the microbes. After incubation the plates were examined for zone of inhibition. Zone of inhibition 22 mm was considered as sensitive and such isolates were excluded from the study, whereas 21 was considered as resistant and reported as a mupirocin(bactroban) resistant MRSA(methillin resistant staphylococcus aureus) and included in the study.

3. Epsilometer test (E-test). The purpose is to determine minimum inhibitory concentration for mupirocin.

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