Biochemistry Question!!! You are working in the lab and discover a new factor (X

Question

Biochemistry Question!!!

You are working in the lab and discover a new factor (X) that appears to play some important role in wound healing. You are provided with the information that each receptor only binds a single X. in order to better characterize the interaction of X with a putative receptor, you obtain response and binding data on X and obtain the following results: These are the data you wish to report: Approximate numbers of X receptor molecules per cell Half-maximal dose. Apparent K_d for the X receptor. Any other details that you think might be important. Write a very concise report containing ALL the information asked and a short paragraph describing why do you think these findings might be interesting.

Explanation / Answer

1. Assume that at at the ligand concentration of 10-8M, the receptors reached saturation and no further increase in proliferation occurred. The graph shows saturation at this point.

So, 12 pmols of ligand is bound to 107 cells, when the receptors are saturated.

12 pmol = 1.2 × 10-11 M

2. Half maximal dose is the concentration of X at which half of the receptors are saturated. From the graph, the half maximal dose value is 10-10M.

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