Biochem: 1. What would happen to the resulting protein if a deamination of an ad

Question

Biochem:

1. What would happen to the resulting protein if a deamination of an adenosine residue was found at the third position of the following anticodon?:

5'-ACA-3'

2. How does intramolecular hydorgen bonding affect tRNA structure and function? What could be a consequence of a mismatched base in this region?

3. Suppose that different mutations in the gene that codes for RNA pol III could cause complete or partial loss of function of this enzyme. Where in the process of DNA=>RNA=>Protein could you possibly see these effects?

4. What are the energy requiring steps of translation? What is the energy requirement to form a 20 chain polypeptide starting with uncharged tRNA and free amino acids?

5. What post-transcriptional modifications play a role in translational efficacy? HOw?

6. Why are there 20 amino acids, 10 aminoacetyl tRNA synthetases, and 64 codons, but the number of tRNA 20-64?

7. A mRNA sequence is provided below followed by mutations in the sequence. Identify the mutations and infer what would happen to the function of the protein.

Original: 5'- CCUAAUGUUUCUUAUUGUAUAG-3'

Mutated: 5'- CCUAAUGUUCUUAUUGGUAUAG-3'

8. A section of a coding strand DNA sequence is provided below. Transcribe this DNA into RNA and then translate it into a protein. Discuss a location where this protein would most likely be found in a cell.

5'-GCATGCGATCTTTCATCCTAGCAGCTAGCTACGAAGATACTAACTACGG-3'

Explanation / Answer

1. The deamination of adenosine at the third position (5'-ACA-3') produces inosine (I) nucleotide, which forms wobble base pair (does not follow Watson - Crick Model of base pairing). Inosine may pair with Uracil or Adenine or Cytosine but not with Guanine.

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