Biochem: 1. Indicate whether the following describes DNA polymerase, RNA polymer

Question

Biochem:

1. Indicate whether the following describes DNA polymerase, RNA polymerase, both, or none:

a. Binds to single stranded DNA

b. Uses an RNA primer

c. Has proof-reading ability

d. Reads from 3' to 5'

e. Phosphodiester bonds are formed using rNTPs

f. Requires a DNA primer

g. Multiple types are present in eukaryotes

h. Mutiple types are present in prokaryotes

i. Is synthesized bi-directionally

j. Requires there to be a terminal sequence on DNA

k. Is part of a larger complex made up of several proteins

2. Post-translational damage causes the loss of the 2' hydoxyl group on several adenosine nucleotides within the intron sequence of a primary RNA transcript. What could possible happen to the resulting mature RNA sequence and consequent protein?

3. Where on the below strand of RNA would you most likely find the following:

a. Start of transcription

b. Start of translation

c. TATA box

d. Enhancers

e. Initiator

f. Activators

g. Repressors

4. How would the following mutation in the template strand of DNA affect the translated protein? (x=any base, / = exon/intron junction)

5-XXXXC/CTGAAGAGGAAGXXXXGTTAGXXXXACTCAC/CTCGC-3 to 5-CCTGAAGAGGAAXXXXXXXXGTTAGXXXXACTCACCTCGC-3

5. When will there be a 5'-5' linkage in RNA? When is this bond formed? Which base is commonly involved in this process? What would happen if there was an inability in this enzyme to make the 5'-5' likage?

6. Consider the following strand of mRNA. What protein would be produced if this strand was translated?

5'- CGUAUGCCCUUUACUAGUAUGUGAUACUAGUAAACC-3'

7. What would be the new protein if the following mutations were made to the strand in 6? Label/classify each type of mutation.

a. 5'-CGUAUGCCCUUUAACUAGUAUGUGAUACUAGUUAACC-3'

b. 5'-CGUAUGCCCUUCACUAGUAUGUGAUACUAGUUAACC-3'

c. 5'- CGUAUGCCCUUUACUAGUAUUGAUACUAGUUAACC-3'

d. 5'- CGUAUGCCCUUGACUAGUAUGUGAUACUAGUAAACC-3'

e. 5'-CGUAUGCCCUUCACUAGUAUGAGUAACUAGUAACC-3'

f. 5'-CGUAUGCCCUAAACUAGUAUGUGAUACUAGUAAACC-3'

8. What are examples of two different types of point mutations in sequences coding for hemoglobin production?

Explanation / Answer

1. Indicate whether the following describes DNA polymerase, RNA polymerase, both, or none:

a. Binds to single stranded DNA.................DNA polymerase

b. Uses an RNA primer................DNA polymerase

c. Has proof-reading ability................DNA polymerase

d. Reads from 3' to 5'................DNA polymerase

e. Phosphodiester bonds are formed using rNTPs................DNA polymerase

f. Requires a DNA primer (NONE)

g. Multiple types are present in eukaryotes................DNA polymerase

h. Mutiple types are present in prokaryotes................DNA polymerase

i. Is synthesized bi-directionally............NONE

j. Requires there to be a terminal sequence on DNA..............(NONE)

k. Is part of a larger complex made up of several proteins................DNA polymerase

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