1. PCR amplification is optimal with the thermostable microbial DNA polymerases
ID: 59803 • Letter: 1
Question
1. PCR amplification is optimal with the thermostable microbial DNA polymerases because
a. they do not require primers for initiation of DNA synthesis
b. they are able to survive the denaturation step
c. they are able to recognize nucleotides
d. they are able to remain active until the denaturation step
2. Thermophoilic microbes are needed for PCR technology because they contain
a. restriction enzymes
b. heat-stable DNA polymerases
c. heat-stable RNA polymerases
d. DNA ligases
e. a useful plasmid vector
3. During a PCR reaction, it is important to use a DNA polymerase that
a. does not require a primer to start DNA synthesis
b. is able to withstand the temperatures used during the denaturation step
c. can synthesize DNA from an RNA template
d. is able to digest non-self DNA
e. has no proofreading capabilities
4. One important feature of a cloning vector is
a. it contains an ori replication
b. it contains a selectable marker, like antibiotic resistance
c. it contains restriction enzymen cleavage sites
d. none of these are important features of a cloning vector
e. all of these are important features of a cloning vector
5. In a restriction-modification system, the methylation system marks the DNA as ___________, while restriction endonuclease degrades __________________.
a. self, methylates DNA
b. foreign, incoming DNA
c. self, foreigh DNA
d. foreign, unmethylated DNA
6. Restriction enymes are used by bacteria for ___________________, but used for cloning for ___________________.
a. DNA replication, preparation of DNA from the source organism
b. transcription, PCR
c. protection against foreign DNA, matcing insert and vector DNA ends
d. limitation of viral infection, selection of proper clones after transformation
7. Which of these is NOT an important feature of a cloning vector?
a. restriction enzyme sites
b. origin of replication
c. Tra functions
d. antibiotic resistance genes
e. all of these are needed in a cloning vector
8. Expressing eukaryotic genes in bacteria is challenging because of the presence of ______________ in many eukaryotic genes,
a. promoters
b. introns
c. exons
d. cDNA
e. insertions
9. A cDNA made from a mature eukaryotic mRNA would lack ______________.
a. introns
b. promoter
c. start codon
d. a and b
e. b and c
10. Bacteria and Archaea are able to distinguish self DNA from foreign DNA. The self DNA is usually differentiated from foreign DNA because self DNA is
a. double-stranded instead of single stranded
b. modified to include a typical nucleotides
c. linear
d. methylated
e. none of the above
11. True/False. You would be expect an archael promoter to be efficiently recognized by the bacterial transcription machinery.
Explanation / Answer
1.PCR amplification is optimal with the thermostable microbial DNA polymerases because
b. they are able to survive the denaturation step
2. Thermophoilic microbes are needed for PCR technology because they contain
b. heat-stable DNA polymerases
Thermus aquaticus is used in PCR.
3.During a PCR reaction, it is important to use a DNA polymerase that
b. is able to withstand the temperatures used during the denaturation step
4. One important feature of a cloning vector is
a. it contains an ori replication
b. it contains a selectable marker, like antibiotic resistance
c. it contains restriction enzymen cleavage sites
5. In a restriction-modification system, the methylation system marks the DNA as ___________, while restriction endonuclease degrades __________________.
6. Restriction enymes are used by bacteria for ___________________, but used for cloning for
c. protection against foreign DNA, matcing insert and vector DNA ends
7. Which of these is NOT an important feature of a cloning vector c. Tra functions
8. Expressing eukaryotic genes in bacteria is challenging because of the presence of ______________ in many eukaryotic genes,
b. introns
9. A cDNA made from a mature eukaryotic mRNA would lack
a. introns
10. Bacteria and Archaea are able to distinguish self DNA from foreign DNA. The self DNA is usually differentiated from foreign DNA because self DNA is
a. double-stranded instead of single stranded
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