Procedure Total Protein Activity Specific Activity | Yield, % mg mmoles/min (mmo

Question

Procedure Total Protein Activity Specific Activity | Yield, % mg mmoles/min (mmoles/min)/mg protein Crude Extract Salt Precipitate Acid Precipitate lon Exchange Affinity Chromatography Size Exclusion 20,000 5,000 4,000 200 50 4 x 106 3 x 106 1 x 106 8 x 105 7.5 x 105 100% 45 6.75X105 Thought Question 8: Determine the Specific Activity for each step in the purification. The original specific activity was: a. 4 x 106 units/mg. b. 100 % units. c. 200 units/mg. d. 0.005 units/mg e. can not be determined

Explanation / Answer

Question 9. Specific activity can be calculated by

Sp = number of units(mL)/Protein Conc in mg/ML

So Answer are

For crude extract Sp = 200

salt precipitation =600

acid ppt = 250

ion exchange = 4000

Affinity chromatography = 15000

size Exclusion = 15000

Question 11 .Ans b) Increased Specific activity

Question 12. Ans Incresed Sp activity

Question 13. Ans a size exclustion chromotography

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