PBS (phosphate buffered saline) is a common salt solution used in a number of pr

Question

PBS (phosphate buffered saline) is a common salt solution used in a number of protocols including protein assays, cell work, etc. The recipe for 10X PBS is as follows:

Dissolve the following into 800 mL distilled H20:

80 g of NaCl (MW = 58.44 g/mol)

2.0 g of KCl (MW = 74.55 g/mol)

2.4 g of KH2PO4 (MW = 136.09 g/mol)

14.4 g of Na2HPO4 (MW = 141.96 g/mol)

Adjust pH to 7.4.

Adjust volume to 1 L with additional distilled H20.

Sterilize by autoclaving.

2) You make 1 L of the 10X stock PBS solution and aliquot it into 10 smaller vials to prevent contamination of your large stock bottle. What is the concentration of each aliquot?

Explanation / Answer

Take 100ml of stock 10x PBS and add 900 ml of distilled water in 1L flask

Like this take 10 times in 10 aliquots

Then it's concentration is 1xPBS of each aliquot

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