Lab, Week # 5 BIOTECHNOLOGY Introduction In this Experiment we will learn how to
ID: 54985 • Letter: L
Question
Lab, Week # 5
BIOTECHNOLOGY
Introduction
In this Experiment we will learn how to identify the unknown bacterium according to the case study given as you start to perform your procedures and following the steps. Also, we will be learning the outcome of a specific case scenario of immunity. We will be performing different examples of media and observing the reaction after inoculation and adding different reagents as it produce some changing of color diagnose to a negative or positive results. Also, we will conduct different types of biochemical testing including Indole production, Citrate Utilization, Methyl red test, and Mannitol Fermentation.
In this case scenario we are trying to figure out the infestation of food at the warehouse causing a death of the plaintiff mother. The plaintiff is accusing the warehouse for selling these infested products by insects. At the warehouse inspection is been done by swabbing and acquiring the type of organism to be identified as evidence for the court final decision.
One of the major findings in this experiment is that it was negative gram stains and the Indole and Methyl Red Test had a positive reaction. Additional testing was perform which show the result of only negative on Lactose Fermentation and the remaining three additional testing add up to a positive. The outcome of this investigation of the bacterium was diagnose to be Shimwellia Blattae.
Procedure
The following steps are requiring completing this lab experimental. First make sure you open the Virtual Unknown Software. Complete conducting using Indole production and Citrate Utilization, Methyl Red test choosing from your media option. Also make sure you open the reference book for each procedure to be informed of the lab steps and decision making such as positive or negative and its color after inoculation. Inoculation of your medium is very important without contamination and placing at the 37 degree incubator temperature and applying the next day option key. Retrieve specimen out of incubator and make your to add the right reagent and observe its color to diagnose and report a negative or positive outcome.
Observations and Results
Result + or -
Appearance after Incubation
Gram Stain
- gram rods
N/A
Indole Production
neg
Yellowish-after incubation & adding Kovac’s reagent no change on its color.
Mannitol Fermentation
- neg
Red
Methyl Red Test
+ pos
Turbid yellowish after incubation and adding methyl red reagent change to red positive. +
Citrate Utilization
- neg
Greenish after incubation. No reagent added.
Additional tests performed are listed below:
Result + or -
Appearance after Incubation
Lactose Fermentation
- neg
Red- no reagent added
Sucrose Fermentation
+ pos
Yellowish- no reagent added
Phenol Red Rhamnose
(Lactose Steps)
+ pos
Yellow- no reagent
Phenol Red Xylose (Sucrose Steps)
+ pos
Yellow- no reagent
Bacterium identified as: You may want to do some additional tests to conclusively identify your bacterium. Some good ones to do might be lactose and sucrose fermentation, like you did last week. Remember, they use Phenol Red Lactose Broth and Phenol Red Sucrose Broth and yellow= positive and red = negative. After you do those two tests, you may want to do Phenol Red Rhamnose and Phenol Red Xylose, which are set up the same way as the sucrose and lactose tests, they just have different sugars. Be sure to record your test results!
The bacteria identifies as Shimwellia Blattae!
Discussion
Identify the bacterium – Make sure you look in the Virtual Unknown lab report to make sure you got the right organism. After you have made your identification, look at the lab report. You can do this by going to the View menu in Virtual Unknown and selecting Lab Report. You can double check with me that you are researching the right one.
My organism suspected to be Shimwellia Blattae due to the Gram-Negative and rod shaped organism.
Explain what features of this organism make it an ideal candidate host for your insecticide. (Hint: remember you will need to infect insects with the bacteria to deliver the insecticide.) –Find out where the unknown organism was isolated from.
Describe in detail the steps you will take to insert the target gene into the bacterium. - Figure 8.1 of your book should help you with this.
The steps to target a gene into the insect is by using the bacteria in the DNA recombinant technology, which we introduce plasmid that carries the gene to the bacteria acquiring cloning and then transmitting the infected insect. The process includes the plasmid to carry the marker selecting it out, to an outcome of insect getting integrated.
Conclusion
In this Biotechnology experiment we apply the knowledge to demonstrate the techniques used industrial and scientific method. In food the founding is that insect that has secreated any example of secretion but, if it goes with food that is completely cooked to a high amount the chance of the outcome to a DNA will be apply and broken. Also, in Biotechnology also use these production to be involve cellular and tissues.
I
Virtual Lab Report
Student: Janet Castano : castano1
Instructor: Test User
Section: Test
Unknown: BiotechUnknown2
Identification Information
Assigned Unknown: Shimwellia blattae
Identified Unknown: Proteus penneri
Gram Reaction & Morphology: Gram Negative Rods
Auto-Inoculation Used: Yes
Test Detail (Chronological Order):
1 Gram Reaction {-} Eliminated {53} Remaining {71} Recorded 1 time(s)
Observations: none
No errors recorded
2 Indole production {-} Eliminated {20} Remaining {51} Recorded 1 time(s)
Observations: none
No errors recorded
3 Mannitol fermentation {-} Eliminated {43} Remaining {8} Recorded 1 time(s)
Observations: none
No errors recorded
4 Methyl red {+} Eliminated {2} Remaining {6} Recorded 1 time(s)
Observations: none
No errors recorded
5 Citrate Utilization (Simmons) {-} Eliminated {0} Remaining {6} Recorded 1 time(s)
Observations: none
No errors recorded
Summary:
Number of Tests Recorded: 5
Number of Media Disposed: 0
Virtual Days: 5
Remaining Microbes: {6}
Date/Time last updated: 2015-10-03 02:55:25.0
Created by
6 Lactose fermentation {-} Eliminated {0} Remaining {6} Recorded 1 time(s)
Observations: none
7 Sucrose fermentation {+} Eliminated {4} Remaining {2} Recorded 1 time(s)
Observations: none
8 Rhamnose fermentation {+} Eliminated {2} Remaining {0} Recorded 1 time(s)
Observations: none
9 Xylose fermentation {+} Eliminated {0} Remaining {0} Recorded
Please help me out responding for the part of Discussion, Also Explain what features of this organism make it an ideal candidate host for your insecticide .Find out where the unknown organism was isolated from? Please help me complete this part that is missing.
Result + or -
Appearance after Incubation
Gram Stain
- gram rods
N/A
Indole Production
neg
Yellowish-after incubation & adding Kovac’s reagent no change on its color.
Mannitol Fermentation
- neg
Red
Methyl Red Test
+ pos
Turbid yellowish after incubation and adding methyl red reagent change to red positive. +
Citrate Utilization
- neg
Greenish after incubation. No reagent added.
Explanation / Answer
Shimwellia blatte is gram negative rod shaped bacterium isolated from hindgut of the cockroach
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