Lab exercise 8-6: A blocking agent, such as dried gelatin, is used to: Select on

Question

Lab exercise 8-6: A blocking agent, such as dried gelatin, is used to:

Select one:

a. Save money versus purchasing high quality Jell-O

b. Last beyond Y2K

c. Bind remaining available sites after the primary antibody has bound the antigen

d. Make it grow big and strong

2/.

Which of the following is not a metabolic product of yogurt culture?

Select one:

a. Glucose

b. Lactate

c. Galactose

d. Acetaldehyde

e. Lactose

3.

Lab Exercise 8-5: Which of the following is NOT an antigen?

Select one:

a. O

b. Rh

c. A

d. B

4.

If you did a Gram-stain instead of a simple stain on the end yogurt product, you would expect to see:

Select one:

a. Microbes would not hold stain

b. Red cocci and rods

c. Red cocci

d. Purple cocci and/or rods

e. Red rods

5.

Lab Exercise 8-6: This experiment with patient serum is performed along side positive controls. If the positive control has a negative result, which of the following is NOT a possible interpretation?

Select one:

a. The sample serum does not contain the antibody

b. The positive control antibodies bound to the blocking agent

c. The positive control antibodies were not added

d. The purified antigen was not added

e. The purified antigen did not bind to the plate wall

Explanation / Answer

[I am answering 1-3 questions asked by you. I hope it helps. Thanks ]

1. Membranes used for blotting techniques shows high affinity for proteins allowing binding and retention of transferred proteins. Before transferred proteins can be detected using antibodies, the membrane must be incubated with a blocking buffer to block the unoccupied binding surfaces on the membrane. Efficient blocking will allow antibodies to access target proteins bound to the membrane. The correct answer is option C.

2. Acetaldehyde is one of the most important aldehydes, occurring widely in nature and being produced on a large scale in industry. It is not a part of yogurt culture. The correct answer is option D.

3. The O blood group does not contain any antigen though it has both A and B antibodies. Hence, it is not an antigen. The correct answer is option A.

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