17.06 1. Match the following vaccine terms 1. Spliced an influenza virus gene en
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Question
17.06
1.
Match the following vaccine terms
1. Spliced an influenza virus gene encoding an internal nucleoprotein into a plasmid that was injected into mice.
2. Genes encoding a part of the protein polysaccharide coat of a virus are spliced into the vaccinia virus genome.
3. A disease that is the result of a single gene defect, that gene therapy has successfully treated.
A. subunit vaccines
B. DNA vaccines
C. X-linked Severe Combined Immunodeficiency Disease (SCID)
2.
DNA vaccines allow health professionals the ability to alert the immune system of invasion via a different arm of the immune system that more naturally attacks invading viruses because
A. whole or internal viral proteins normally never seen by the immune system will be instructed to express on cell surfaces, where the immune system can notice them more easily.
B. outer coat proteins of a pathogenic virus will be expressed by a harmless virus.
C. small fragments of viral proteins that are not immunogenic nor protective will be carried in to cells by harmless virus carriers.
D. the entire virus is "spliced" (by recombinant DNA techniques) into a vector to be expressed by the cells, where it create mutant versions of itself with the hosts genomic DNA.
3.
Gene therapies can have deleterious side effects because
A. extra body parts can result from overexpression of recombinant DNA.
B. cells will hijack this foreign DNA and use it to overthrow all safeguards to become an alien 6 foot virus.
C. when foreign DNA is introduced into cellular nuclear DNA it can affect other DNA/protein's expression, and in some circumstances, lead to cancers.
D. the foreign DNA can mutate and replace all normal DNA.
4.
Gene therapy can be used for diseases for which we are not sure which genes are responsible.
True
False
5.
Match the following molecular technique terms.
1. An animal or plant containing a gene that has been introduced without the use of conventional breeding.
2. The inactivation of a specific gene to assess the effect of the loss of its function in an organism.
3. Cells derived from early embryos that can develop into different adult tissues.
4. The ability to create base-pair substitutions at any site in a cloned gene to examine the effect on its function.
A. knockout
B. transgenic
C. in vitro mutagenesis
D. embryonic stem cells
6.
Insulin was one of the first drugs to be produced by transgenic bacteria.
True
False
17.07
7.
In attempts to confer special characteristics upon plants, genetic engineers find Ti (tumor-inducing) plasmid of Agrobacterium tumefaciens to be an effective vector for use with
A. soybeans.
B. corn.
C. rice.
D. barley.
E. wheat.
8.
Which of the following organisms were used in the production of "golden rice" which contains pro-vitamin A, to alleviate Vitamin A deficiencies in children around the world?
A. other varieties of domesticated rice.
B. other varieties of wild rice.
C. carrots.
D. daffodils, corn, and bacteria.
9.
Animals are not affected by Bt toxin in plant crops because
A. animals lack the enzymes to interact with it.
B. while it is toxic to insects, it is a normal metabolite for animals.
C. it is destroyed by acids in the stomach.
D. animals have the enzymes to convert it to a nontoxic substance.
E. animals do not produce aromatic amino acids so Bt toxin cannot be incorporated into metabolic pathways.
10.
Match the following recombinant plant terms.
1. A biodegradable herbicide that kills most actively growing plants by inhibiting EPSP synthetase.
2. A piece of DNA that other genes have been attached to, which can integrate into the DNA of broadleaf plants.
3. The use of transgenic plants for medicinal or pharmaceutical purposes.
A. biopharming
B. Ti (tumor inducing) plasmid
C. glyphosphate
11.
If peanut genes that code for proteins that have nothing to do with peanut allergies in people, are "spliced" into, or recombined with, the DNA of corn plants to make them more drought resistant, children who are allergic to peanuts might suffer a fatal allergic reaction upon eating this corn.
True
False
Explanation / Answer
1.
Spliced an influenza virus gene encoding an internal nucleoprotein into a plasmid that was injected into mice - A. subunit vaccines.
Genes encoding a part of the protein polysaccharide coat of a virus are spliced into the vaccinia virus genome - B. DNA vaccines
A disease that is the result of a single gene defect, that gene therapy has successfully treated - X-linked Severe Combined Immunodeficiency Disease (SCID).
2.
DNA vaccines allow health professionals the ability to alert the immune system of invasion via a different arm of the immune system that more naturally attacks invading viruses because outer coat proteins of a pathogenic virus will be expressed by a harmless virus. Option B is correct.
3.
Gene therapies can have deleterious side effects because when foreign DNA is introduced into cellular nuclear DNA it can affect other DNA/protein's expression, and in some circumstances, lead to cancers. Option C is correct.
4.
Gene therapy can be used for diseases for which we are not sure which genes are responsible is false. This is because genetherapy can be initiated only when the information about the gene is available.
5.
An animal or plant containing a gene that has been introduced without the use of conventional breeding -- B. transgenic
The inactivation of a specific gene to assess the effect of the loss of its function in an organism -- A. knockout.
Cells derived from early embryos that can develop into different adult tissues -- embryonic stem cells.
The ability to create base-pair substitutions at any site in a cloned gene to examine the effect on its function -- in vitro mutagenesis
6.
Insulin was one of the first drugs to be produced by transgenic bacteria is True.
7.
In attempts to confer special characteristics upon plants, genetic engineers find Ti (tumor-inducing) plasmid of Agrobacterium tumefaciens to be an effective vector for use with corn becaue, the corn or maize posesses transposable elements. Option B is correct.
8.
In the production of "golden rice" which contains pro-vitamin A, to alleviate Vitamin A deficiencies in children around the world daffodils, corn, and bacteria were used. Option D is correct.
9.
Animals are not affected by Bt toxin in plant crops because while it is toxic to insects, it is a normal metabolite for animals. Option B is correct.
10.
A biodegradable herbicide that kills most actively growing plants by inhibiting EPSP synthetase -- C. glyphosphate
A piece of DNA that other genes have been attached to, which can integrate into the DNA of broadleaf plants -- B. Ti (tumor inducing) plasmid
The use of transgenic plants for medicinal or pharmaceutical purposes -- A. biopharming.
11.
The given statement is false because those genes are not responsible to cause peanut allergies.
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