17.04 1. Match the following 1. Annealing of a complementary single-stranded pro
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Question
17.04
1.
Match the following
1. Annealing of a complementary single-stranded probe to identify specific DNA's in complex mixtures.
2. A collection of many different stretches of DNA in a vector that together represents the entire genome of an organism.
3. An enzyme isolated from retroviruses that can make cDNA from an mRNA template.
4. A collection of many different types of DNA that each represents the genes expressed as proteins in many different tissues or cells.
A. reverse transcriptase
B. genomic library
C. hybridization
D. cDNA Library
2.
How is a cDNA library different from a genomic library?
A. cDNA does not contain introns.
B. cDNA contains RNA nucleotides.
C. the cDNA library contains sequences not found in the genomic library.
D. cDNA libraries can be screened by hybridization while genomic libraries cannot.
E. cDNA is not double stranded while genomic DNA is.
3.
A representative genomic library of an organism's overlapping DNA fragments results from the use of
A. viruses that cleave the DNA into random pieces.
B. many pieces of recombinant DNA from several different types of organisms.
C. partial digestion with frequently cutting restriction endonucleases.
D. DNA ligase joining multiple types of vectors.
E. random pieces of DNA that are made such that they never overlap.
4.
In screening for clones containing specific DNA genomic library pieces, it is common to use
A. milipore filters randomly impregnated with dyes or radiation.
B. solutions that will always maintain cellular membrane integrity.
C. antibiotics.
D. complementary viruses.
E. molecular hybridization with probes.
5.
When wishing to create an expression library that will express proteins from a eukaryotic cell, in a bacterial host, using mRNA and reverse transcriptase solves the problem of
A. alternative splicing.
B. post-translational modifications.
C. transformation of foreign DNA.
D. nucleic acid base pair matching.
17.05
6.
Which of the following is an example of reverse genetics?
A. finding a mouse gene can direct eye formation in Drosophila
B. identifying a protein-protein interaction by a yeast two-hybrid screen
C. isolating clones from a library using hybridization of a radiolabeled probe
D. observing a phenotype and isolating the gene that produces that phenotype
E. mutating a cloned gene and substituting the mutant copy for the wild-type copy in an organism to assess its function
7.
One of the most useful methods for identifying a specific DNA sequence is
A. magnetic resonance imaging.
B. thin-layer chromatography.
C. the Western blot.
D. the Southern blot.
E. the Eastern blot.
8.
Match the following molecular techniques.
1. Acts as a chain terminator when incorporated into the DNA because it lacks an OH at the 3' position.
2. Point mutations found in a population that can eliminate or create restriction sites.
3. Allows the amplification of a single DNA fragment from a small amount of a complex mixture of DNA.
4. A technique in which proteins are separated by electrophoresis and probed with a specific antibody.
A. polymerase chain reaction
B. dideoxynucleotides
C. western blot
D. restriction fragment length polymorphisms
9.
Which of the following would be best as a probe in a Southern blot?
A. A double stranded fragment identical to the target sequence.
B. A single stranded fragment complementary to the target sequence.
C. A single stranded fragment identical to the target sequence.
D. A double stranded fragment complementary to the target sequence.
17.06
10.
In making a "piggyback" vaccine for herpes (one virus carrying coat proteins of another), the gene for an identity marker of the herpes virus is spliced into the genome of the ____ virus, which was discovered 200 years ago by Edward Jenner as a result of his work to vaccinate against smallpox.
A. smallpox
B. chickenpox
C. cowpox
D. rabbitpox
E. opossum pox
Explanation / Answer
1.1C2B3A4D
2.A
3.C
4.E
5.B
6.A
7.D
8.1B2D3A4C
9.B
10.A (SMALLPOX)
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