You have two samples of DNA, sample 1 has a GC content of 60% and is dissolved i

Question

You have two samples of DNA, sample 1 has a GC content of 60% and is dissolved
in a buffer with 10 mM salt concentration. Sample 2 has a GC content of 30% and is
dissolved in a buffer with 100 mM salt concentration. If the length of DNA is the
same in both, which one has a higher melting temperature? Use the following formula
to calculate Tm. Tm = 81.5 + 16.6 log[M] + 0.41(%GC) - 675/L
1) Which sample has the higher Tm? Sample 1 or 2?
2) What is the difference in the melting Temp between the two samples? _____ C.

Explanation / Answer

Calculate the Tm for sample1:

Tm = 81.5+16.6 log[M]+0.41(%GC)-675/L

= 81.5+16.6 log[10]+0.41(60)-675

= 225.63

Calculate the Tm for sample2:

= 81.5+16.6 log[100]+0.41(30)-675

= 451.26

2. The difference is

= 451.26-225.63

= 225.63

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