You have two different C. elegans mutant lines. One line has a mutation in a gen

Question

You have two different C. elegans mutant lines. One line has a mutation in a gene called daf-2 and the other has a mutation in a gene called daf-16. The daf-6 mutant is short lived and the daf-2 mutant is long-lived. To determine if the two genes work in the same gene regulatory pathway, you perform an epistasis analysis and find that daf-2, daf-16 double mutants are short-lived.

a) Draw a gene regulatory pathway to explain these results.

b) You mutagenize wild type C. elegans and find two more mutants that result in increased lifespan. You call one age-1 and the other akt-1. age-1, daf-16 double mutants and akt-1, daf-16 double mutants are long lived. Draw all possible gene regulatory pathways for daf-2, daf-17, akt-1, and age-1 based on these data.

Explanation / Answer

Based on the given data,

a)

For development, metabolism, longevity and stress respose the DAF-2 (a receptor) and ADF-16 (Forkhead transcription factor) are essentially requried. In the given conditon the daf-6+ (mutant) is short lived and daf-2 (mutant) is long lived. The F1 daf-2, daf-16+ double mutants are short-lived. This states that daf-6+ is masking the expression of daf-2.

The gene regulatory pathway for both questions is as follows:

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