Figure #7 depicts the mechanism of Topoisomerase and its DNA substracte: After t

Question

Figure #7 depicts the mechanism of Topoisomerase and its DNA substracte: After taking a good look at the image above, answer the following questions Which domain(s) of this protein most likely exhibits helicase like activity? Explain. Which domain(s) of this protein most likely exhibits endonuclease activity? Explain. Which domain(s) of this protein has the highest affinity for single stranded DNA? Explain. Which domain(s) of this protein would likely exhibit large patches of positively charged amino acids? Explain. Topoisomerase is said to oscillate between an open conformation and a closed conformation, which panels (a-f) do you think represent each of the two possible conformations?

Explanation / Answer

a) Domain I actually exhibits helicase like activity. This is clearly depicted in the first image (a), where domain I binds to DNA and breaks the bonds between two DNA strands.

b) Domain III and IV exhibits endonuclease activity. This is depicted in the diagram (b).

c) Domains III and IV of the protein has the highest affinity for single stranded DNA.

d) Domains III and IV have the largest patches of positively charged amino acids, and they involved in the biding of DNA, which is negatively charged.

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